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Title (Primary) Metabolic transformation as a diagnostic tool for the selection of candidate promutagens in effect-directed analysis
Author Hug, C.; Krauss, M.; Nüsser, L.; Hollert, H.; Brack, W.;
Journal Environmental Pollution
Year 2015
Department WANA;
Volume 196
Language englisch;
POF III (all) T42;
Keywords Ames fluctuation test; Effect-directed analysis; Candidate exclusion; Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
UFZ wide themes RU3;
Abstract To implement metabolic activation by S9 rat liver homogenate in the selection of candidate promutagens in effect-directed analysis, we critically assessed the capability of LC-HRMS measurements to detect depletion and formation of metabolites by S9 exposure. The exposure of a reference mixture to S9 led to a depletion by >70% for most compounds. Other processes than metabolism were excluded as significant contribution to compound depletion. Metabolites formed by S9 exposure were identified and S9 metabolism was incorporated in the identification of candidate promutagens in a wastewater treatment plant (WWTP) effluent with mutagenic activity only after metabolic activation by S9. The metabolism by S9 in the WWTP effluent was confirmed. Based on a candidate exclusion of all peaks not depleted, thus not activated by the S9 mix, the number of candidate promutagens was reduced by 40%. Selected remaining candidates were evaluated and identified, but could not be confirmed as promutagens.
ID 15447
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15447
Hug, C., Krauss, M., Nüsser, L., Hollert, H., Brack, W. (2015):
Metabolic transformation as a diagnostic tool for the selection of candidate promutagens in effect-directed analysis
Environ. Pollut. 196 , 114 - 124