Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Evaluation of toluene degradation pathways by two-dimensional stable isotope fractionation
Author Vogt, C.; Cyrus, E.; Herklotz, I.; Schlosser, D.; Bahr, A.; Herrmann, S.; Richnow, H.-H.; Fischer, A.;
Journal Environmental Science & Technology
Year 2008
Department ISOBIO; UMB; UBT;
Volume 42
Issue 21
Language englisch;
Abstract Toluene degradation by several pure and mixed microbial cultures was investigated by two-dimensional compound specific isotope analysis (2D-CSIA). For most of the cultures, the respective toluene degradation pathway and toluene attacking enzymatic step was known. The slope of the linear regression for hydrogen (Delta delta H-2)vs carbon (Delta delta C-13) discrimination (Lambda =Delta delta H-2/ Delta delta C-13 approximate to epsilon H-bulk/epsilon C-bulk) was determined in order to characterize aerobic and anaerobic toluene degradation pathways. The highest A value was estimated for the monohydroxylation of the methyl group by Pseudomonas putida (Lambda = 53 +/- 5). The lowest value was observed for Rhodococcus opacus (Lambda = 2 +/- 2) due to its insignificant hydrogen fractionation, which indicates that a ring dioxygenase was responsible for the initial attack of toluene. The fungus Cladosprium sphaerospermum containing a cytochrome P450-dependent methyl monooxygenase gr! ouped within these extreme values (Lambda = 16 +/- 6). A values for organisms attacking toluene under anoxic conditions by benzylsuccinate synthase were significantly different and ranged from Lambda = 4 +/- 3 (Blastochloris sulfoviridis) to 31 +/- 11 (strain TRM1). Values were in the same range for organisms using nitrate (Lambda = 11-14) or sulfate (Lambda = 28-31) as electron acceptor, indicating that it might be possible to distinguish toluene degradation under different electron acceptor conditions by 2D-CSIA.
ID 1539
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1539
Vogt, C., Cyrus, E., Herklotz, I., Schlosser, D., Bahr, A., Herrmann, S., Richnow, H.-H., Fischer, A. (2008):
Evaluation of toluene degradation pathways by two-dimensional stable isotope fractionation
Environ. Sci. Technol. 42 (21), 7793 - 7800