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Title (Primary) Methods for visualising active microbial toluene and benzene degraders in in situ microcosms
Author Schurig, C.; Müller, C.W.; Höschen, C.; Prager, A.; Kothe, E.; Beck, H.; Miltner, A.; Kästner, M.;
Journal Applied Microbiology and Biotechnology
Year 2015
Department UBT;
Volume 99
Issue 2
Language englisch;
POF III (all) T41;
Keywords NanoSIMS; 13C; Microbial activity; In situ microcosms ; DP-BACTRAPs; Benzene
UFZ wide themes RU3;
Abstract Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microcosm approach, so-called ‘direct-push bacterial trap’ (DP-BACTRAP), with nano-scale secondary ion mass spectrometry (NanoSIMS) analysis on samples from contaminated constructed wetlands. This approach is based on initially sterile microcosms amended with 13C-labelled benzene as a source of carbon and energy for microorganisms. The microcosms were introduced directly in the constructed wetland, where they were colonised by indigenous microorganisms from the sediment. After incubation in the field, the samples were analysed by NanoSIMS, scanning electron microscopy (SEM) and fluorescence microscopy in order to visualise 13C-labelled microbial biomass on undisturbed samples from the microcosms. With the approach developed, we successfully visualised benzene-degrading microbes on solid materials with high surface area by means of NanoSIMS. Moreover, we could demonstrate the feasibility of NanoSIMS analysis of unembedded porous media with a highly complex topography, which was frequently reasoned to not lead to sufficient results.
ID 15286
Persistent UFZ Identifier
Schurig, C., Müller, C.W., Höschen, C., Prager, A., Kothe, E., Beck, H., Miltner, A., Kästner, M. (2015):
Methods for visualising active microbial toluene and benzene degraders in in situ microcosms
Appl. Microbiol. Biotechnol. 99 (2), 957 - 968