Publication Details

Reference Category Book chapters
DOI / URL link
Title (Primary) Isolation and analysis of membrane phospholipids as biomarkers
Title (Secondary) Hydrocarbon and lipid microbiology protocols. Biochemical methods
Author Hachicho, N.; Heipieper, H.J.;
Publisher McGenity, T.J.; Timmis, K.N.; Nogales, B.;
Journal Springer Protocols Handbooks
Year 2016
Department UBT;
Language englisch;
POF III (all) T41;
Keywords Biodiversity; Biomarkers; Community analysis; Microbial ecology; Phospholipid fatty acids (PLFA); Stress response
UFZ wide themes RU3;
Abstract The protocol describes the analysis and data interpretation of membrane phospholipid fatty acid (PLFA) profiles as biomarkers for the characterisation of microbial communities in environmental samples, with special emphasis on soils and sediments contaminated with organic hydrocarbons. This simple method in microbial ecology studies provides an estimate of the viable microbial biomass, first indications of the structure of the microbial community, as well as the physiological status of the microbiota. In environmental biotechnological applications, PLFA analysis is a useful tool for monitoring bioremediation and allows direct validation of existing biological activities as well as hints for shifts in the microbiota caused by successful biological treatment strategies.
ID 15278
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15278
Hachicho, N., Heipieper, H.J. (2016):
Isolation and analysis of membrane phospholipids as biomarkers
In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.)
Hydrocarbon and lipid microbiology protocols. Biochemical methods
Springer Protocols Handbooks
Springer, Berlin, Heidelberg, p. 123 - 132