Publication Details |
Category | Text Publication |
Reference Category | Book chapters |
DOI | 10.1007/8623_2014_17 |
Title (Primary) | Isolation and analysis of membrane phospholipids as biomarkers |
Title (Secondary) | Hydrocarbon and lipid microbiology protocols. Biochemical methods |
Author | Hachicho, N.; Heipieper, H.J. |
Publisher | McGenity, T.J.; Timmis, K.N.; Nogales, B. |
Source Titel | Springer Protocols Handbooks |
Year | 2016 |
Department | UBT |
Page From | 123 |
Page To | 132 |
Language | englisch |
Keywords | Biodiversity; Biomarkers; Community analysis; Microbial ecology; Phospholipid fatty acids (PLFA); Stress response |
UFZ wide themes | RU3; |
Abstract | The protocol describes the analysis and data interpretation of membrane phospholipid fatty acid (PLFA) profiles as biomarkers for the characterisation of microbial communities in environmental samples, with special emphasis on soils and sediments contaminated with organic hydrocarbons. This simple method in microbial ecology studies provides an estimate of the viable microbial biomass, first indications of the structure of the microbial community, as well as the physiological status of the microbiota. In environmental biotechnological applications, PLFA analysis is a useful tool for monitoring bioremediation and allows direct validation of existing biological activities as well as hints for shifts in the microbiota caused by successful biological treatment strategies. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15278 |
Hachicho, N., Heipieper, H.J. (2016): Isolation and analysis of membrane phospholipids as biomarkers In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.) Hydrocarbon and lipid microbiology protocols. Biochemical methods Springer Protocols Handbooks Springer, Berlin, Heidelberg, p. 123 - 132 10.1007/8623_2014_17 |