Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1016/j.bpc.2014.08.002 |
Title (Primary) | A thermodynamic investigation of the glucose-6-phosphate isomerization |
Author | Hoffmann, P.; Held, C.; Maskow, T.
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Source Titel | Biophysical Chemistry |
Year | 2014 |
Department | UMB |
Volume | 195 |
Page From | 22 |
Page To | 31 |
Language | englisch |
Keywords | Thermodynamic equilibrium constant; Activity coefficient; Gibbs energy of reaction; Enthalpy of reaction; ePC-SAFT; Glycolysis |
UFZ wide themes | RU3; |
Abstract | In this work, ΔRg+ values for the enzymatic G6P isomerization were determined as a function of the G6P equilibrium molality between 25 °C and 37 °C. The reaction mixtures were buffered at pH = 8.5. In contrast to standard literature work, ΔRg+ values were determined from activity-based equilibrium constants instead of molality-based apparent values. This yielded a ΔRg+ value of 2.55 ± 0.05 kJ mol− 1 at 37 °C, independent of the solution pH between 7.5 and 8.5. Furthermore, ΔRh+ was measured at pH = 8.5 and 25 °C yielding 12.05 ± 0.2 kJ mol− 1. Accounting for activity coefficients turned out to influence ΔRg+ up to 30% upon increasing the G6P molality. This result was confirmed by predictions using the thermodynamic model ePC-SAFT. Finally, the influence of the buffer and of potassium glutamate as an additive on the reaction equilibrium was measured and predicted with ePC-SAFT in good agreement. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15274 |
Hoffmann, P., Held, C., Maskow, T., Sadowski, G. (2014): A thermodynamic investigation of the glucose-6-phosphate isomerization Biophys. Chem. 195 , 22 - 31 10.1016/j.bpc.2014.08.002 |