Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1128/JCM.00552-14 |
| Title (Primary) | Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites |
| Author | Krohn, S.; Böhm, S.; Engelmann, C.; Hartmann, J.; Brodzinski, A.; Chatzinotas, A.; Zeller, K.; Prywerek, D.; Fetzer, I.; Berg, A. |
| Journal | Journal of Clinical Microbiology |
| Year | 2014 |
| Department | UMB |
| Volume | 52 |
| Issue | 5 |
| Page From | 1754 |
| Page To | 1757 |
| Language | englisch |
| UFZ wide themes | RU3; |
| Abstract | Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml−1. Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Gram-positive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15071 |
| Krohn, S., Böhm, S., Engelmann, C., Hartmann, J., Brodzinski, A., Chatzinotas, A., Zeller, K., Prywerek, D., Fetzer, I., Berg, A. (2014): Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites J. Clin. Microbiol. 52 (5), 1754 - 1757 |
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