Publication Details

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Title (Primary) Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites
Author Krohn, S.; Böhm, S.; Engelmann, C.; Hartmann, J.; Brodzinski, A.; Chatzinotas, A.; Zeller, K.; Prywerek, D.; Fetzer, I.; Berg, A.;
Journal Journal of Clinical Microbiology
Year 2014
Department UMB;
Volume 52
Issue 5
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml−1. Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Gram-positive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples.
ID 15071
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15071
Krohn, S., Böhm, S., Engelmann, C., Hartmann, J., Brodzinski, A., Chatzinotas, A., Zeller, K., Prywerek, D., Fetzer, I., Berg, A. (2014):
Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites
J. Clin. Microbiol. 52 (5), 1754 - 1757