Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Experimental results and integrated modelling of bacterial growth on insoluble hydrophobic substrate (phenanthrene)
Author Adam, I.K.U.; Rein, A.; Miltner, A.; Fulgêncio, A.C.D.; Trapp, S.; Kästner, M.;
Journal Environmental Science & Technology
Year 2014
Department UBT;
Volume 48
Issue 15
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract Metabolism of a low-soluble substrate is limited by dissolution and availability and can hardly be determined. We developed a numerical model simultaneously calculating dissolution kinetics of such substrates, its metabolism and microbial growth (Monod kinetics with decay) and tested it with three aerobic phenanthrene (PHE) degraders: Novosphingobium pentaromativorans US6-1, Sphingomonas sp. EPA505 and Sphingobium yanoikuyae B1. PHE was present as microcrystals, providing non-limiting conditions for growth. Total PHE and protein concentration were tracked over 6 to 12 days. The model was fitted to the test results for the rates of dissolution, metabolism and growth. The strains showed similar efficiency, with vmax values of 12 to 18 g dw g-1 d-1, yields of 0.21 g g-1, maximum growth rates of 2.5 to 3.8 d-1 and decay rates of 0.04 to 0.05 d-1. Sensitivity analysis with the model shows that i) retention in crystals, NAPLs or by sequestration compete with biodegradation, ii) bacterial growth conditions (dissolution flux and resulting chemical activity of substrate) are more relevant for the final state of the system than the initial biomass; and iii) the desorption flux regulates the turnover in the presence of solid-state, sequestered (aged) or NAPL substrate sources.
ID 15020
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15020
Adam, I.K.U., Rein, A., Miltner, A., Fulgêncio, A.C.D., Trapp, S., Kästner, M. (2014):
Experimental results and integrated modelling of bacterial growth on insoluble hydrophobic substrate (phenanthrene)
Environ. Sci. Technol. 48 (15), 8717 - 8726