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Title (Primary) A quantitative HPLC–MS/MS method for studying internal concentrations and toxicokinetics of 34 polar analytes in zebrafish (Danio rerio) embryos
Author Brox, S.; Ritter, A.P.; Küster, E.; Reemtsma, T.;
Journal Analytical and Bioanalytical Chemistry
Year 2014
Department BIOTOX; ANA;
Volume 406
Issue 20
Language englisch;
POF III (all) T42;
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-014-7929-y/MediaObjects/216_2014_7929_MOESM1_ESM.pdf
Keywords Bioconcentration; Toxicodynamic; Absorption, distribution, metabolism and excretion; Larvae; Electrospray; Toxicity
UFZ wide themes RU3;
Abstract An analytical method using high-performance liquid chromatography–tandem mass spectrometry was developed to determine internal concentrations of 34 test compounds such as pharmaceuticals and pesticides in zebrafish embryos (ZFE), among them, cimetidine, 2,4-dichlorophenoxyacetic acid, metoprolol, atropine and phenytoin. For qualification and quantification, multiple reaction monitoring mode was used. The linear range extends from 0.075 ng/mL for thiacloprid and metazachlor and 7.5 ng/mL for coniine and clofibrate to 250 ng/mL for many of the test compounds. Matrix effects were strongest for nicotine, but never exceeded ±20 % for any of the developmental stages of the ZFE. Method recoveries ranged from 90 to 110 % from an analysis of nine pooled ZFE. These findings together with the simple sample preparation mean this approach is suitable for the determination of internal concentrations from only nine individual ZFE in all life stages up to 96 h post-fertilization. Exemplarily, the time course of the internal concentrations of clofibric acid, metribuzin and benzocaine in ZFE was studied over 96 h, and three different patterns were distinguished, on the basis of the speed and extent of uptake and whether or not a steady state was reached. Decreasing internal concentrations may be due to metabolism in the ZFE.
ID 14983
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14983
Brox, S., Ritter, A.P., Küster, E., Reemtsma, T. (2014):
A quantitative HPLC–MS/MS method for studying internal concentrations and toxicokinetics of 34 polar analytes in zebrafish (Danio rerio) embryos
Anal. Bioanal. Chem. 406 (20), 4831 - 4840