Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/1462-2920.12510 |
| Document | Shareable Link |
| Title (Primary) | Development and application of primers for the class Dehalococcoidia (phylum Chloroflexi) enables deep insights into diversity and stratification of sub-groups in the marine subsurface |
| Author | Wasmund, K.; Algora, C.; Müller, J.; Krüger, M.; Lloyd, K.G.; Reinhardt, R.; Adrian, L. |
| Source Titel | Environmental Microbiology |
| Year | 2015 |
| Department | ISOBIO; BIOTOX |
| Volume | 17 |
| Issue | 10 |
| Page From | 3540 |
| Page To | 3556 |
| Language | englisch |
| Supplements | https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0001-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0002-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0003-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0004-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0005-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0006-si.pdf https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.12510&file=emi12510-sup-0007-si.doc |
| Keywords | marine; sediment; subsurface; Dehalococcoidia; Chloroflexi; diversity; real-time PCR; pyrosequencing |
| UFZ wide themes | RU3; |
| Abstract | Bacteria of the class Dehalococcoidia (DEH) (phylum Chloroflexi) are widely distributed in the marine subsurface and are especially prevalent in deep marine sediments. Nevertheless, little is known about the specific distributions of DEH sub-groups at different sites and depths. This study therefore specifically examined the distributions of DEH through depths of various marine sediment cores by quantitative PCR and pyrosequencing using newly designed DEH 16S rRNA gene targeting primers. Quantification of DEH showed populations may establish in shallow sediments (i.e., upper centimetres) although as low relative proportions of total Bacteria, yet often became more prevalent in deeper sediments. Pyrosequencing revealed pronounced diversity co-exists within single biogeochemical zones, and that clear and sometimes abrupt shifts in relative proportions of DEH sub-groups occur with depth. These shifts indicate varying metabolic properties exist among DEH sub-groups. The distributional changes in DEH sub-groups with depth may be related to a combination of biogeochemical factors including the availability of electron acceptors such as sulphate and the composition of organic matter and depositional regimes. Collectively, the results suggest DEH exhibit wider metabolic and genomic diversity than previously recognised, and this contributes to their widespread occurrence in the marine subsurface. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14932 |
| Wasmund, K., Algora, C., Müller, J., Krüger, M., Lloyd, K.G., Reinhardt, R., Adrian, L. (2015): Development and application of primers for the class Dehalococcoidia (phylum Chloroflexi) enables deep insights into diversity and stratification of sub-groups in the marine subsurface Environ. Microbiol. 17 (10), 3540 - 3556 10.1111/1462-2920.12510 |
|