Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) A PCR-based assay for the detection of anaerobic naphthalene degradation
Author Morris, B.E.L.; Gissibl, A.; Kümmel, S.; Richnow, H.-H.; Boll, M.
Journal FEMS Microbiology Letters
Year 2014
Department ISOBIO
Volume 354
Issue 1
Page From 55
Page To 59
Language englisch
Keywords polycyclic aromatic hydrocarbons; PCR-based assay; anaerobic aromatic degradation; 2-naphthoyl-CoA reductase
UFZ wide themes RU3;
Abstract Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of considerable risk to human health. The aerobic degradation of PAH via oxygenase reactions has been studied for several decades. In contrast, it was not until very recent that the first key enzyme involved in anaerobic PAH degradation, the dearomatizing 2-naphthoyl-CoA reductase, was isolated and characterized. In this work, a PCR-based functional assay was developed to detect microorganisms that have the ability to anaerobically degrade naphthalene, as a model for larger PAH. The degenerative oligonucleotide probes introduced here amplified a highly conserved region of the gene encoding 2-naphthoyl-CoA reductase (Ncr) in numerous sulfate-reducing pure cultures and environmental enrichments. The assay provides the first molecular tool for monitoring the anaerobic degradation of a model PAH.
Persistent UFZ Identifier
Morris, B.E.L., Gissibl, A., Kümmel, S., Richnow, H.-H., Boll, M. (2014):
A PCR-based assay for the detection of anaerobic naphthalene degradation
FEMS Microbiol. Lett. 354 (1), 55 - 59