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Title (Primary) Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
Author Ramírez-Cavazos, L.I.; Junghanns, C.; Nair, R.; Cárdenas-Chávez, D.L.; Hernández-Luna, C.; Agathos, S.N.; Parra, R.;
Journal Journal of Zhejiang University-Science B
Year 2014
Department ZENCO;
Volume 15
Issue 4
Language englisch;
POF III (all) T41;
Keywords Central composite design; Copper sulfate; Enhanced laccase production; Inducers; Screening media; Soybean oil; Tomato juice medium
UFZ wide themes RU3;
Abstract The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate (CuSO4). The best conditions obtained from screening experiments in shaken flasks using tomato juice, CuSO4, and soybean oil were integrated in an experimental design. Enhanced levels of tomato juice as the medium, CuSO4 and soybean oil as inducers (36.8% (v/v), 3 mmol/L, and 1% (v/v), respectively) were determined for 10 L stirred tank bioreactor runs. This combination resulted in laccase titer of 143 000 IU/L (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 3.0), which represents the highest activity so far reported for P. sanguineus in a 10-L fermentor. Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20 000 IU/L, whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times. Based on a partial characterization, the laccases of this strain are especially promising in terms of thermostability (half-life of 6.1 h at 60 °C) and activity titers.
ID 14800
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14800
Ramírez-Cavazos, L.I., Junghanns, C., Nair, R., Cárdenas-Chávez, D.L., Hernández-Luna, C., Agathos, S.N., Parra, R. (2014):
Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
J. Zhejiang Univ.-SCI. B 15 (4), 343 - 352