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Title (Primary) Are rice (Oryza sativa L.) phosphate transporters regulated similarly by phosphate and arsenate? A comprehensive study
Author Muehe, E.M.; Eisele, J.A.; Daus, B.; Kappler, A.; Harter, K.; Chaban, C.
Journal Plant Molecular Biology
Year 2014
Department ANA
Volume 85
Issue 3
Page From 301
Page To 316
Language englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs11103-014-0186-9/MediaObjects/11103_2014_186_MOESM1_ESM.doc
Keywords Arsenic; Arsenate; Phosphate transporters; Rice (Oryza sativa); Gene regulation; Growth inhibition
UFZ wide themes RU4;
Abstract Rice is one of the most important staple foods worldwide, but it often contains inorganic arsenic, which is toxic and gives rise to severe health problems. Rice plants take up arsenate As(V) via the phosphate transport pathways, though it is not known how As(V), as compared to phosphate, modifies the expression of phosphate transporters (PTs). Therefore, the impact of As(V) or phosphate (Pi) on the gene expression of PTs and several Pi signaling regulators was investigated. Rice plants were grown on medium containing different As(V) or Pi concentrations. Growth was evaluated and the expression of tested genes was quantified at different time points, using quantitative RT-PCR (qPCR). The As and P content in plants was determined using inductively coupled plasma mass spectrometry (ICP-MS). As(V) elicited diverse and opposite responses of different PTs in roots and shoots, while Pi triggered a more shallow and uniform transcriptional response in several tested genes. Only a restricted set of genes, including PT2, PT3, PT5 and PT13 and two SPX-MFS family members, was particularly responsive to As(V). Despite some common reactions, the responses of the analyzed genes were predominantly ion-specific. The possible reasons and consequences are discussed.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14765
Muehe, E.M., Eisele, J.A., Daus, B., Kappler, A., Harter, K., Chaban, C. (2014):
Are rice (Oryza sativa L.) phosphate transporters regulated similarly by phosphate and arsenate? A comprehensive study
Plant Mol.Biol. 85 (3), 301 - 316