Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1080/01490450902754441
Title (Primary) Predominance of Methanolobus spp. and Methanoculleus spp. in the archaeal communities of saline gas field formation fluids
Author Ehinger, S.; Seifert, J.; Kassahun, A.; Schmalz, L.; Hoth, N.; Schlömann, M.
Source Titel Geomicrobiology Journal
Year 2009
Department PROTEOM
Volume 26
Issue 5
Page From 326
Page To 338
Language englisch
Keywords Biogeochemistry; methanogens; prokaryote diversity; subsurface microbiology
Abstract The microbial communities in sulfate-rich, saline formation fluids of a natural gas reservoir in Lower Saxony, Germany were investigated to enhance the knowledge about microbial communities in potential carbon dioxide sequestration sites. This investigation of the initial state of the deep subsurface microbiota is necessary to predict their influence on the long-term stability and storage capacity of such sites. While the bacterial 16S rDNA gene library was comprised of sequences affiliating with the Firmicutes, the Alphaproteobacteria, the Gammaproteobacteria and the Thermotogales, the archaeal 16S rDNA libraries were simply dominated by two phylotypes related to the genera Methanolobus and Methanoculleus. The monitoring of the archaeal communities in different formation fluid samples by T-RFLP and Real-Time-PCR indicated that these two methanogenic genera dominated at all, whereas the proportion of the two groups varied. Thus, methylotrophic and autotrophic methanogenesis seems to be of importance in the reservoir fluids, dependent on the provided reduction equivalents and substrates and it also may influence the fate of CO2 in the subsurface.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=146
Ehinger, S., Seifert, J., Kassahun, A., Schmalz, L., Hoth, N., Schlömann, M. (2009):
Predominance of Methanolobus spp. and Methanoculleus spp. in the archaeal communities of saline gas field formation fluids
Geomicrobiol. J. 26 (5), 326 - 338 10.1080/01490450902754441