Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.mimet.2008.05.015
Title (Primary) In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH
Author Shiraishi, F.; Zippel, B.; Neu, T.R.; Arp, G.
Journal Journal of Microbiological Methods
Year 2008
Department FLOEK
Volume 75
Issue 1
Page From 103
Page To 108
Language englisch
Keywords FISH; CARD-FISH; Tyramide signal amplification (TSA); Stromatolite; Mineralized biofilm
Abstract Modified protocols of fluorescence in situ hybridization (FISH) and catalyze reporter deposition fluorescence in situ hybridization (CARD-FISH) were developed in order to detect bacteria in situ in calcified stromatolite biofilms. Smooth, well-preserved thin sections of calcified biofilms (~ 5 ┬Ám thin, vertical sectioning of ~ 1 cm deep) were obtained by cryo-sectioning using the adhesive tape-stabilization technique. A modified hybridization buffer was applied during hybridization to prevent calcite dissolution as well as false binding of oligonucleotide probes to the charged mineral surfaces. Particularly, bright and specific CARD-FISH signals allowed the detection of bacteria in intensively calcified biofilms even at low magnification, which is suitable for investigating millimeter- to centimeter-scale vertical distribution patterns of bacteria.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1458
Shiraishi, F., Zippel, B., Neu, T.R., Arp, G. (2008):
In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH
J. Microbiol. Methods 75 (1), 103 - 108