Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.mimet.2008.05.015 |
| Title (Primary) | In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH |
| Author | Shiraishi, F.; Zippel, B.; Neu, T.R.; Arp, G. |
| Source Titel | Journal of Microbiological Methods |
| Year | 2008 |
| Department | FLOEK |
| Volume | 75 |
| Issue | 1 |
| Page From | 103 |
| Page To | 108 |
| Language | englisch |
| Keywords | FISH; CARD-FISH; Tyramide signal amplification (TSA); Stromatolite; Mineralized biofilm |
| Abstract | Modified protocols of fluorescence in situ hybridization (FISH) and catalyze reporter deposition fluorescence in situ hybridization (CARD-FISH) were developed in order to detect bacteria in situ in calcified stromatolite biofilms. Smooth, well-preserved thin sections of calcified biofilms (~ 5 µm thin, vertical sectioning of ~ 1 cm deep) were obtained by cryo-sectioning using the adhesive tape-stabilization technique. A modified hybridization buffer was applied during hybridization to prevent calcite dissolution as well as false binding of oligonucleotide probes to the charged mineral surfaces. Particularly, bright and specific CARD-FISH signals allowed the detection of bacteria in intensively calcified biofilms even at low magnification, which is suitable for investigating millimeter- to centimeter-scale vertical distribution patterns of bacteria. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1458 |
| Shiraishi, F., Zippel, B., Neu, T.R., Arp, G. (2008): In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH J. Microbiol. Methods 75 (1), 103 - 108 10.1016/j.mimet.2008.05.015 |
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