Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.phytochem.2013.11.023 |
| Title (Primary) | Identification of UGT84A13 as a candidate enzyme for the first committed step of gallotannin biosynthesis in pedunculate oak (Quercus robur) |
| Author | Mittasch, J.; Böttcher, C.; Frolova, N.; Bönn, M.; Milkowski, C. |
| Source Titel | Phytochemistry |
| Year | 2014 |
| Department | BOOEK |
| Volume | 99 |
| Page From | 44 |
| Page To | 51 |
| Language | englisch |
| Keywords | Quercus robur; Fagaceae; Gallotannins; Glucosyltransferase; Substrate specificity |
| UFZ wide themes | RU1 |
| Abstract | A cDNA encoding the ester-forming hydroxybenzoic acid glucosyltransferase UGT84A13 was isolated from a cDNA library of Quercus robur swelling buds and young leaves. The enzyme displayed high sequence identity to resveratrol/hydroxycinnamate and hydroxybenzoate/hydroxycinnamate glucosyltransferases from Vitis species and clustered to the phylogenetic group L of plant glucosyltransferases, mainly involved in the formation of 1-O-β-d-glucose esters. In silico transcriptome analysis confirmed expression of UGT84A13 in Quercus tissues which were previously shown to exhibit UDP-glucose:gallic acid glucosyltransferase activity. UGT84A13 was functionally expressed in Escherichia coli as N-terminal His-tagged protein. In vitro kinetic measurements with the purified recombinant enzyme revealed a clear preference for hydroxybenzoic acids as glucosyl acceptor in comparison to hydroxycinnamic acids. Of the preferred in vitro substrates, protocatechuic, vanillic and gallic acid, only the latter and its corresponding 1-O-ß-D-glucose ester were found to be accumulated in young oak leaves. This indicates that in planta UGT84A13 catalyzes the formation of , 1-O-galloyl-ß-D-glucose, the first committed step of gallotannin biosynthesis. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14408 |
| Mittasch, J., Böttcher, C., Frolova, N., Bönn, M., Milkowski, C. (2014): Identification of UGT84A13 as a candidate enzyme for the first committed step of gallotannin biosynthesis in pedunculate oak (Quercus robur) Phytochemistry 99 , 44 - 51 10.1016/j.phytochem.2013.11.023 |
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