Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s10973-013-3494-4 |
| Document | Shareable Link |
| Title (Primary) | Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile |
| Author | Morais, F.M.; Buchholz, F.; Hartmann, T.; Lerchner, J.; Neu, T.R.; Kiesel, B.; Harms, H.; Maskow, T.
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| Source Titel | Journal of Thermal Analysis and Calorimetry |
| Year | 2014 |
| Department | UMB; FLOEK |
| Volume | 115 |
| Issue | 3 |
| Page From | 2203 |
| Page To | 2210 |
| Language | englisch |
| Keywords | Chip-calorimetry; Biofilm; Bacteriophage; Phage infection; Microbial interaction; Pseudomonas putida |
| UFZ wide themes | RU2; |
| Abstract | Bacteriophages or phage-derived biological structures are a promising alternative to the application of antibiotics to eradicate biofilms. These countermeasures are highly cell specific. For a better understanding of the sequence of the underlying processes (attachment, infection, multiplication, phage release), for optimization of phage applications, or simply for screening of suitable phages or phage-derived enzymes, real-time monitoring devices are urgently required. Calorimetry is promising because it is non-invasive and quantitatively connected to the metabolic fluxes. Chip-calorimetry provides real-time information about biofilm eradication by phages. This was confirmed by comparison with reference analyses (i.e., confocal laser scanning microscopy, colony plate counts, or phage titre determination). Furthermore, chip-calorimetry provides additional information which was not captured by the reference methods such as the enhanced cellspecific heat production caused by the infection process and a residual activity of seemingly persistent bacteria. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14210 |
| Morais, F.M., Buchholz, F., Hartmann, T., Lerchner, J., Neu, T.R., Kiesel, B., Harms, H., Maskow, T. (2014): Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile J. Therm. Anal. Calorim. 115 (3), 2203 - 2210 10.1007/s10973-013-3494-4 |
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