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Title (Primary) Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile
Author Morais, F.M.; Buchholz, F.; Hartmann, T.; Lerchner, J.; Neu, T.R.; Kiesel, B.; Harms, H.; Maskow, T.;
Journal Journal of Thermal Analysis and Calorimetry
Year 2014
Department UMB; FLOEK;
Volume 115
Issue 3
Language englisch;
POF III (all) T32; T41;
Keywords Chip-calorimetry; Biofilm; Bacteriophage; Phage infection; Microbial interaction; Pseudomonas putida
UFZ wide themes RU2;
Abstract Bacteriophages or phage-derived biological structures are a promising alternative to the application of antibiotics to eradicate biofilms. These countermeasures are highly cell specific. For a better understanding of the sequence of the underlying processes (attachment, infection, multiplication, phage release), for optimization of phage applications, or simply for screening of suitable phages or phage-derived enzymes, real-time monitoring devices are urgently required. Calorimetry is promising because it is non-invasive and quantitatively connected to the metabolic fluxes. Chip-calorimetry provides real-time information about biofilm eradication by phages. This was confirmed by comparison with reference analyses (i.e., confocal laser scanning microscopy, colony plate counts, or phage titre determination). Furthermore, chip-calorimetry provides additional information which was not captured by the reference methods such as the enhanced cellspecific heat production caused by the infection process and a residual activity of seemingly persistent bacteria.
ID 14210
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14210
Morais, F.M., Buchholz, F., Hartmann, T., Lerchner, J., Neu, T.R., Kiesel, B., Harms, H., Maskow, T. (2014):
Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile
J. Therm. Anal. Calorim. 115 (3), 2203 - 2210