Publication Details

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Title (Primary) Starvation of Jurkat T cells causes metabolic switch from glycolysis to lipolysis as revealed by comprehensive GC-qMS
Author Tomm, J.M.; Otto, W.; Servos, U.; Bertram, B.; von Bergen, M.; Baumann, S.;
Journal Journal of Integrated OMICS
Year 2013
Department METABOX; PROTEOM;
Volume 3
Issue 1
Language englisch;
POF III (all) F11;
UFZ wide themes RU3;
Abstract

T cells play a central role in the cellular part of the immune system and their metabolomic activity is strictly bound to the status of activation. Little is known about the metabolomic resilience of T helper cells in the resting status. Therefore we analyzed the metabolomic profile of non-activated Jurkat T cells under normal and starvation conditions by a two-dimensional GC-MS approach. We detected 52 organic and amino acids in the biological replicates covering the majority of central metabolic pathways. Under starvation conditions 21 analytes representing major metabolic pathways showed a significant down-regulation. For palmitoleic acid a significant up-regulation was detected. The annotation of differentially abundant metabolites to the pathways of TCA-cycle, amino acid metabolism and fatty acid biosynthesis indicating a metabolic switch from glycolytic to lipolytic energy generation upon starvation.

ID 13995
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13995
Tomm, J.M., Otto, W., Servos, U., Bertram, B., von Bergen, M., Baumann, S. (2013):
Starvation of Jurkat T cells causes metabolic switch from glycolysis to lipolysis as revealed by comprehensive GC-qMS
Journal of Integrated OMICS 3 (1), 70 - 74