Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1061/(ASCE)EE.1943-7870.0000711 |
| Title (Primary) | Biodiversity of polyphosphate accumulating bacteria in eight WWTPs with different modes of operation |
| Author | Mehlig, L.; Petzold, M.; Heder, C.; Günther, S.; Müller, S.; Eschenhagen, M.; Röske, I.; Röske, K. |
| Source Titel | Journal of Environmental Engineering-ASCE |
| Year | 2013 |
| Department | UMB |
| Volume | 139 |
| Issue | 8 |
| Page From | 1089 |
| Page To | 1098 |
| Language | englisch |
| UFZ wide themes | ru3 |
| Abstract | Enhanced biological phosphorous removal (EBPR) from wastewater has
been successfully used for more than three decades and is considered to be an
environmentally friendly wastewater-treatment process. Biologically, this
process is realized by incorporation of phosphate as polyphosphate (polyP)
granules in activated sludge bacteria. Important groups of bacteria responsible
for P removal have been identified, but the full microbial diversity involved in
this process is still unknown. This paper reports on the microbial composition
of activated sludge communities in eight wastewater-treatment plants (WWTPs)
with different sizes and modes of operation. The polyphosphate accumulating
organisms (PAOs) within this complex biocenosis were identified by fluorescent
dye staining and classified by in situ hybridization techniques. Of the bacteria
in the aerobic basin, 5–13% contained polyP granules. In addition, flow
cytometry was used to quantify PAOs after tetracycline staining and to separate
these cells. The phylogenetic affiliation of the sorted PAOs was identified by
cloning and sequencing. Both workflows showed similar outcomes. The majority of
PAOs in all plants were Betaproteobacteria (22%), Actinobacteria
(21%), and Alphaproteobacteria (12%), with differences in the relative
abundance. In addition, Bacteroidetes (12%) were detected in the clone
libraries, especially Haliscomenobacter, which should be considered
further with regard to its influence on the EBPR process. The denaturing
gradient gel electrophoresis analyses of sorted PAOs revealed a diverse
community composition of Betaproteobacteria, Actinobacteria, and
Rhodocyclales in the WWTPs. PAOs were present in EBPR and non-EBPR WWTPs,
and no correlation in their abundance and phylogenetic composition to the mode
of operation was revealed. This study shows that specific PAO communities
existed in the various WWTPs, probably favored by the respective wastewater
composition, including so far unvalued PAOs species, but their active
contribution in the EBPR process remains to be
investigated. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13918 |
| Mehlig, L., Petzold, M., Heder, C., Günther, S., Müller, S., Eschenhagen, M., Röske, I., Röske, K. (2013): Biodiversity of polyphosphate accumulating bacteria in eight WWTPs with different modes of operation J. Environ. Eng.-ASCE 139 (8), 1089 - 1098 10.1061/(ASCE)EE.1943-7870.0000711 |
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