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Title (Primary) Sulfur-34S stable isotope labeling of amino acids for quantification (SULAQ34) of proteomic changes in Pseudomonas fluorescens during naphthalene degradation
Author Herbst, F.-A.; Taubert, M.; Jehmlich, N.; Behr, T.; Schmidt, F.; von Bergen, M.; Seifert, J.;
Journal Molecular & Cellular Proteomics
Year 2013
Department PROTEOM;
Volume 12
Issue 8
Language englisch;
POF III (all) F11; T41;
Keywords Bioinformatics software; Cell biology*; Mass Spectrometry; Prokaryotes*; Quantification
UFZ wide themes RU3;
Abstract The relative quantification of proteins is one of the major techniques to elucidate physiological reactions. In order to avoid artifacts due to chemical labeling the metabolic introduction of heavy isotopes into proteins and peptides is the preferred method for relative quantification. For eukaryotic cells stable isotope labeling by amino acids in cell culture (SILAC) has become the gold standard which can be readily applied to a vast number of scenarios. In the microbial realm with its highly versatile metabolic capabilities SILAC is often not feasible and using other 13C or 15N labeled substrates may not be practical. Here, the incorporation of heavy sulfur isotopes is a useful alternative. We therefore introduce 34sulfur stable isotope labeling of amino acids for quantification (SULAQ34) and the corresponding tools required for spectra extraction and disintegration of the isotopic overlaps caused by the small mass shift. As a proof of principle we investigated the proteomic changes related to naphthalene degradation in P. fluorescens ATCC 17483 and uncovered a specific oxidative stress like response.
ID 13673
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13673
Herbst, F.-A., Taubert, M., Jehmlich, N., Behr, T., Schmidt, F., von Bergen, M., Seifert, J. (2013):
Sulfur-34S stable isotope labeling of amino acids for quantification (SULAQ34) of proteomic changes in Pseudomonas fluorescens during naphthalene degradation
Mol. Cell. Proteomics 12 (8), 2060 - 2069