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Title (Primary) The internal concentration of organic substances in fish embryos - a toxicokinetic approach
Author Kühnert, A.; Vogs, C.; Altenburger, R.; Küster, E.;
Journal Environmental Toxicology and Chemistry
Year 2013
Department BIOTOX;
Volume 32
Issue 8
Language englisch;
POF III (all) T42;
Keywords Internal dose; Toxicity assessment; Bioaccumulation; 3R; Body burden
UFZ wide themes RU3;
Abstract In ecotoxicity assessment, the ambient exposure concentration is typically applied to quantify the toxic potential of xenobiotic substances. However, exposure and organism-related differences in bioconcentration often cause a considerable variability of toxicity data. This can be minimized by using the internal organism concentration, because toxicokinetic modifying factors are considered implicitly. In this study, the relationship between ambient and internal concentration-time profiles was investigated for zebrafish (Danio rerio) embryos. The aim was to gain a better understanding and interpretation of exposure based-methods using this model organism. For this purpose, a simple and effective approach to determine the internal concentration was developed. Embryos were exposed to a series of four neutral organic substances (naphthalene, fluorene, fluoranthene, benz[a]anthracene) of different hydrophobicity for 72 hours. The internal and ambient concentrations were measured at eight to nine time points. Kinetics of uptake and elimination were modeled using a first-order one-compartment model. Biotransformation processes appeared to influence the internal concentrations of fluoranthene and benz[a]anthracene after 48 hours. The bioconcentration factors (BCF) obtained are in excellent agreement with those determined in previous studies using radiolabelled substances. The method demonstrated here is a further step towards a refined ecotoxicity assessment using fish embryos, which links toxicity to the chemical concentration within the organism. This system may also be considered as an alternative to animal testing for BCF determination.
ID 13648
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Kühnert, A., Vogs, C., Altenburger, R., Küster, E. (2013):
The internal concentration of organic substances in fish embryos - a toxicokinetic approach
Environ. Toxicol. Chem. 32 (8), 1819 - 1827