Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.copbio.2012.10.017
Title (Primary) Subpopulation-proteomics in prokaryotic populations
Author Jahn, M.; Seifert, J.; von Bergen, M.; Schmid, A.; Bühler, B.; Müller, S.
Source Titel Current Opinion in Biotechnology
Year 2013
Department UMB; PROTEOM
Volume 24
Issue 1
Page From 79
Page To 87
Language englisch
UFZ wide themes RU3;

Clonal microbial cells do not behave in an identical manner and form subpopulations during cultivation. Besides varying micro-environmental conditions, cell inherent features like cell cycle dependent localization and concentration of regulatory proteins as well as epigenetic properties are well accepted mechanisms creating cell heterogeneity. Another suspected reason is molecular noise on the transcriptional and translational level. A promising tool to unravel reasons for cell heterogeneity is the combination of cell sorting and subpopulation proteomics. This review summarizes recent developments in prokaryotic single-cell analytics and provides a workflow for selection of single cells, low cell number mass spectrometry, and proteomics evaluation. This approach is useful for understanding the dependency of individual cell decisions on inherent protein profiles.
Persistent UFZ Identifier
Jahn, M., Seifert, J., von Bergen, M., Schmid, A., Bühler, B., Müller, S. (2013):
Subpopulation-proteomics in prokaryotic populations
Curr. Opin. Biotechnol. 24 (1), 79 - 87 10.1016/j.copbio.2012.10.017