Publication Details

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Title (Primary) Subpopulation-proteomics in prokaryotic populations
Author Jahn, M.; Seifert, J.; von Bergen, M.; Schmid, A.; Bühler, B.; Müller, S.;
Journal Current Opinion in Biotechnology
Year 2013
Department UMB; PROTEOM;
Volume 24
Issue 1
Language englisch;
POF III (all) F11; T41;
UFZ wide themes RU3;
Abstract

Clonal microbial cells do not behave in an identical manner and form subpopulations during cultivation. Besides varying micro-environmental conditions, cell inherent features like cell cycle dependent localization and concentration of regulatory proteins as well as epigenetic properties are well accepted mechanisms creating cell heterogeneity. Another suspected reason is molecular noise on the transcriptional and translational level. A promising tool to unravel reasons for cell heterogeneity is the combination of cell sorting and subpopulation proteomics. This review summarizes recent developments in prokaryotic single-cell analytics and provides a workflow for selection of single cells, low cell number mass spectrometry, and proteomics evaluation. This approach is useful for understanding the dependency of individual cell decisions on inherent protein profiles.
ID 13116
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13116
Jahn, M., Seifert, J., von Bergen, M., Schmid, A., Bühler, B., Müller, S. (2013):
Subpopulation-proteomics in prokaryotic populations
Curr. Opin. Biotechnol. 24 (1), 79 - 87