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Title (Primary) Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil
Author Peršoh, D.; Theuerl, S.; Buscot, F.; Rambold, G.;
Journal Journal of Microbiological Methods
Year 2008
Department BOOEK;
Volume 75
Issue 1
Language englisch;
Keywords DNA; RNA; Nucleic acid extraction; Soil
Abstract A universally adaptable protocol for quantitative extraction of high-purity nucleic acids from soil is presented. A major problem regarding the extraction of nucleic acids from soil is the presence of humic substances, which interfere with the extraction process itself and in subsequent analytical manipulations. By the approach described here, the humic compounds are precipitated prior to cell lysis with Al2(SO4)3, and thus eliminated prior to the nucleic acid extraction. The protocol allows for removing of a considerable content and range of humic acids and should therefore be applicable for a wide spectrum of soil types. Accordingly, reproducible results in analyses of different soil types are made possible, inclusively for quantitative comparisons.
ID 1306
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1306
Peršoh, D., Theuerl, S., Buscot, F., Rambold, G. (2008):
Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil
J. Microbiol. Methods 75 (1), 19 - 24