Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Single nucleotide primer extension assay for detection and sequence typing of Dehalococcoides spp.
Author Nikolausz, M.; Chatzinotas, A.; Palatinszky, M.; Imfeld, G.; Martínez, P.; Kästner, M.;
Journal Applied and Environmental Microbiology
Year 2008
Department ISOBIO; UMB; UBT;
Volume 74
Issue 1
Language englisch;
Abstract A single nucleotide primer extension (SNuPE) assay in combination with taxon-specific 16S rRNA gene PCR was developed for the detection and typing of Dehalococcoides populations. Specificity of the assay was evaluated with an isolate and an environmental 16S rRNA gene sequence representing two Dehalococcoides subgroups, i.e. the Pinellas and the Cornell subgroups. Only one sequence type belonging to the Pinellas subgroup was detected in a Bitterfeld/Wolfen region aquifer containing chlorinated ethenes as main contaminants. The three primers hybridization assay thus provided a fast and easy to implement confirmation of the specificity of taxon-specific PCR and allowed a rapid additional taxonomic affiliation into subgroups. This study demonstrates the great potential of SNuPE as a novel approach for rapid parallel detection and typing of different nucleic acid signature sequences from environmental samples.
ID 1266
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1266
Nikolausz, M., Chatzinotas, A., Palatinszky, M., Imfeld, G., Martínez, P., Kästner, M. (2008):
Single nucleotide primer extension assay for detection and sequence typing of Dehalococcoides spp.
Appl. Environ. Microb. 74 (1), 300 - 304