|Title (Primary)||Production of a robust nanobiocatalyst for municipal wastewater treatment|
|Author||Hommes, G.; Gasser, C.A.; Howald, C.B.C.; Goers, R.; Schlosser, D.; Shahgaldian, P.; Corvini, P.F.-X.|
|Keywords||laccase; fumed silica nanoparticles; immobilization; phenolic micropollutant; wastewater treatment|
Immobilization is a fundamental method to improve both enzyme activity and stability. In the present work, the process previously described for immobilizing laccase – an enzyme oxidizing phenolic compounds – onto fumed silica was optimized, in order to efficiently produce industrially relevant amounts of a nanobiocatalyst for biological micropollutant elimination, whilst saving 80% of surface modification agent (3-aminopropyl triethoxy silane) and 90% of cross-linker (glutaraldehyde). Minimized losses during preparation and favorable effects of immobilization yielded conjugates with drastically increased enzymatic activity (164% of invested activity). Long-term stability and activity regarding bisphenol A (2,2-bis(4-hydroxyphenyl)propane) removal of the synthesized biocatalyst were assessed under application-relevant conditions. With 81.1 ± 0.4% residual activity after 7 days, stability of conjugates was drastically higher than of free laccase, which showed virtually no activity after 1.5 days.
These results illustrate the huge potential of fumed silica nanoparticles/laccase-composites for innovative biological wastewater treatment.
|Persistent UFZ Identifier||https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12585|
|Hommes, G., Gasser, C.A., Howald, C.B.C., Goers, R., Schlosser, D., Shahgaldian, P., Corvini, P.F.-X. (2012):
Production of a robust nanobiocatalyst for municipal wastewater treatment
Bioresour. Technol. 115 (July 2012), 8 - 15