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Title (Primary) Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica
Author Solé, M.; Müller, I.; Pecyna, M.J.; Fetzer, I.; Harms, H.; Schlosser, D.;
Journal Applied and Environmental Microbiology
Year 2012
Department UMB;
Volume 78
Issue 13
Language englisch;
Abstract

To advance the understanding of the molecular mechanisms controlling microbial activities involved in carbon cycling and mitigation of environmental pollution in freshwaters, the influence of heavy metals and natural as well as xenobiotic organic compounds on laccase gene expression was quantified using quantitative RT-PCR in an exclusively aquatic fungus (the aquatic hyphomycete Clavariopsis aquatica) for the first time. Five putative laccase genes (lcc1 to lcc5) identified in C. aquatica were differentially expressed in response to the fungal growth stage and potential laccase inducers, with certain genes being up-regulated by e.g. the lignocellulose breakdown product vanillic acid, the endocrine disruptor technical nonylphenol, manganese, and zinc. lcc4 is inducible by vanillic acid and most likely encodes for an extracellular laccase already excreted during the trophophase of the organism, suggesting a function during fungal substrate colonization. Surprisingly, unlike many laccases of terrestrial fungi, none of the C. aquatica laccase genes was found to be up-regulated by copper. However, copper strongly increases extracellular laccase activity in C. aquatica, possibly due to stabilisation of the copper-containing catalytic centre of the enzyme. Copper was found to half-saturate laccase activity already at about 1.8 μM, in favour of a fungal adaptation to low copper concentrations of aquatic habitats.

ID 12404
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12404
Solé, M., Müller, I., Pecyna, M.J., Fetzer, I., Harms, H., Schlosser, D. (2012):
Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica
Appl. Environ. Microb. 78 (13), 4732 - 4739