Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1111/j.1574-6941.2012.01334.x |
Title (Primary) | Functional analysis of an anaerobic m-xylene-degrading enrichment culture using protein-based stable isotope probing |
Author | Bozinovski, D.; Herrmann, S.; Richnow, H.-H.; von Bergen, M.; Seifert, J.; Vogt, C. |
Journal | FEMS Microbiology Ecology |
Year | 2012 |
Department | ISOBIO; PROTEOM |
Volume | 81 |
Issue | 1 |
Page From | 134 |
Page To | 144 |
Language | englisch |
Keywords | Desulfobacteriaceae;Epsilonproteobacteria;benzylsuccinate synthase;hydrocarbon degradation |
Abstract |
A sulfate-reducing consortium maintained for several years in the laboratory with m-xylene as sole source of carbon and energy was characterized by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of PCR-amplified 16S rRNA genes and stable isotope probing of proteins (Protein-SIP). During growth upon m-xylene or methyl-labeled m-xylene (1,3-dimethyl-13C2-benzene), a phylotype affiliated to the family Desulfobacteriaceae became most abundant. A second dominant phylotype was affiliated to the phylum Epsilonproteobacteria. In cultures grown with methyl-labeled m-xylene, 331 proteins were identified by LC-MS/MS analysis. These proteins were either not 13C-labeled (23%) or showed a 13C-incorporation of 19–22 atom% 13C (77%), the latter demonstrating that methyl groups of m-xylene were assimilated. 13C-labeled proteins were involved in anaerobic m-xylene biodegradation, in sulfate reduction, in the Wood-Ljungdahl-pathway, and in general housekeeping functions. Thirty-eight percent of the labeled proteins were affiliated to Deltaproteobacteria. Probably due to a lack of sequence data from Epsilonproteobacteria, only 14 proteins were assigned to this phylum. Our data suggest that m-xylene is assimilated by the Desulfobacteriaceae phylotype, whereas the role of the Epsilonproteobacterium in the consortium remained unclear. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12361 |
Bozinovski, D., Herrmann, S., Richnow, H.-H., von Bergen, M., Seifert, J., Vogt, C. (2012): Functional analysis of an anaerobic m-xylene-degrading enrichment culture using protein-based stable isotope probing FEMS Microbiol. Ecol. 81 (1), 134 - 144 |