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Title (Primary) Functional analysis of an anaerobic m-xylene-degrading enrichment culture using protein-based stable isotope probing
Author Bozinovski, D.; Herrmann, S.; Richnow, H.-H.; von Bergen, M.; Seifert, J.; Vogt, C.;
Journal FEMS Microbiology Ecology
Year 2012
Department ISOBIO; PROTEOM;
Volume 81
Issue 1
Language englisch;
Keywords Desulfobacteriaceae;Epsilonproteobacteria;benzylsuccinate synthase;hydrocarbon degradation
Abstract

A sulfate-reducing consortium maintained for several years in the laboratory with m-xylene as sole source of carbon and energy was characterized by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of PCR-amplified 16S rRNA genes and stable isotope probing of proteins (Protein-SIP). During growth upon m-xylene or methyl-labeled m-xylene (1,3-dimethyl-13C2-benzene), a phylotype affiliated to the family Desulfobacteriaceae became most abundant. A second dominant phylotype was affiliated to the phylum Epsilonproteobacteria. In cultures grown with methyl-labeled m-xylene, 331 proteins were identified by LC-MS/MS analysis. These proteins were either not 13C-labeled (23%) or showed a 13C-incorporation of 19–22 atom% 13C (77%), the latter demonstrating that methyl groups of m-xylene were assimilated. 13C-labeled proteins were involved in anaerobic m-xylene biodegradation, in sulfate reduction, in the Wood-Ljungdahl-pathway, and in general housekeeping functions. Thirty-eight percent of the labeled proteins were affiliated to Deltaproteobacteria. Probably due to a lack of sequence data from Epsilonproteobacteria, only 14 proteins were assigned to this phylum. Our data suggest that m-xylene is assimilated by the Desulfobacteriaceae phylotype, whereas the role of the Epsilonproteobacterium in the consortium remained unclear.

ID 12361
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12361
Bozinovski, D., Herrmann, S., Richnow, H.-H., von Bergen, M., Seifert, J., Vogt, C. (2012):
Functional analysis of an anaerobic m-xylene-degrading enrichment culture using protein-based stable isotope probing
FEMS Microbiol. Ecol. 81 (1), 134 - 144