Publication Details
Category Text Publication
Reference Category Journals
DOI 10.1099/mic.0.059402-0
Title (Primary) A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of KlMGA2 gene in Kluyveromyces lactis
Author Micolonghi, C.; Ottaviano, D.; Di Silvio, E.; Damato, G.; Heipieper, H.J. ORCID logo ; Bianchi, M.M.
Source Titel Microbiology-SGM
Year 2012
Department UBT
Volume 158
Issue 7
Page From 1734
Page To 1744
Language englisch
Abstract

In the respiratory yeast Kluyveromyces lactis, little is known about the factors regulating the metabolic response to oxygen shortage. After searching for homologs of characterized Saccharomyces cerevisiae regulators of the hypoxic response, we identified a gene that we named KlMGA2, homologous to MGA2. The deletion of KlMGA2 strongly reduced both fermentative and respiratory growth rate and altered fatty acid composition and unsaturation index of membranes. The reciprocal heterologous expression of MGA2 and KlMGA2 in the corresponding deletion mutant strains suggested that Mga2 and KlMga2 are functional homologs. KlMGA2 transcription was induced by hypoxia and the glucose sensor Rag4 mediated the hypoxic induction of KlMGA2. Transcription of lipid biosynthetic genes KlOLE1, KlERG1, KlFAS1 and KlATF1 was induced by hypoxia and was dependent on KlMga2, except for KlOLE1. Rag4 was required for hypoxic induction of transcription for both KlMga2 dependent (KlERG1) and KlMga2 independent (KlOLE1) structural genes.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12301
Micolonghi, C., Ottaviano, D., Di Silvio, E., Damato, G., Heipieper, H.J., Bianchi, M.M. (2012):
A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of KlMGA2 gene in Kluyveromyces lactis
Microbiology-(UK) 158 (7), 1734 - 1744 10.1099/mic.0.059402-0