Publication Details

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Title (Primary) Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria
Author Buchholz, F.; Lerchner, J.; Mariana, F.; Kuhlicke, U.; Neu, T.R.; Harms, H.; Maskow, T.;
Journal Biofouling
Year 2012
Department UMB; FLOEK;
Volume 28
Issue 3
Language englisch;
Keywords biofilm; calorimetry; biological control agent; Bdellovibrio; predator–prey interaction
Abstract

Control or removal of undesired biofilms has frequently been found to be quite difficult. In addition to biocidal or antibiotic chemicals or materials designed to prevent biofouling, biological control agents appear to be promising. Reports of bacterial predators eradicating biofilms or eliminating pathogens motivate a more systematic screening of biofilm-eliminating bacterial predators. Unfortunately, the analysis of the eradication process is demanding. In the present study, chip-calorimetry was applied to monitor the elimination of Pseudomonas sp. biofilms by Bdellovibrio bacteriovorus. The method uses metabolic heat as a real-time parameter for biofilm activity. The method is non-invasive, fast and convenient due to real-time data acquisition. In addition, heat-production data can reveal information about the energetics of the predator–prey interaction. The calorimetric results were validated by confocal laser scanning microscopy. The approach described may be useful for the screening of biofilm susceptibility to different predators.
ID 12277
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12277
Buchholz, F., Lerchner, J., Mariana, F., Kuhlicke, U., Neu, T.R., Harms, H., Maskow, T. (2012):
Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria
Biofouling 28 (3), 351 - 362