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Title (Primary) Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE): site characterization and on-site bioremediation
Author Fayolle-Guichard, F.; Durand, J.; Cheucle, M.; Rosell, M.; Michelland, R.J.; Tracol, J.-P.; Le Roux, F.; Grundman, G.; Atteia, O.; Richnow, H.H.; Dumestre, A.; Benoit, Y.;
Journal Journal of Hazardous Materials
Year 2012
Department ISOBIO;
Volume 201-202
Language englisch;
Keywords Ethyl tert-butyl ether (ETBE); Groundwater; Bioaugmentation; Carbon isotopic fractionation; ethB gene; Quantitative real-time PCR (q-PCR)
Abstract

Ethyl tert-butyl ether (ETBE) was detected at high concentration (300 mg L−1) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE: 0.91 and 0.83 mg L−1 h−1 and BTEX: 0.64 and 0.82 mg L−1 h−1, respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74 mg L−1). An on-site pilot plant (2 m3) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5 × 106 copies of ethB gene per L−1.
ID 12175
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12175
Fayolle-Guichard, F., Durand, J., Cheucle, M., Rosell, M., Michelland, R.J., Tracol, J.-P., Le Roux, F., Grundman, G., Atteia, O., Richnow, H.H., Dumestre, A., Benoit, Y. (2012):
Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE): site characterization and on-site bioremediation
J. Hazard. Mater. 201-202 , 236 - 243