|Title (Primary)||Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae)|
|Author||Michalski, S.G.; Durka, W.|
|Journal||American Journal of Botany|
|Keywords||454 sequencing; inbreeding; Juncaceae; Juncus effusus; wetland|
• Premise of the study: Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity.
• Methods and Results: Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former.
• Conclusions: The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species.
|Persistent UFZ Identifier||https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12130|
|Michalski, S.G., Durka, W. (2012):
Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae)
Am. J. Bot. 99 (2), e53 - e55