Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1128/AEM.06568-11
Title (Primary) Calcite biomineralization by bacterial isolates from the recently discovered pristine karstic Herrenberg Cave
Author Rusznyák, A.; Akob, D.M.; Nietzsche, S.; Eusterhues, K.; Totsche, K.U.; Neu, T.R.; Frosch, T.; Popp, J.; Keiner, R.; Geletneky, J.; Katzschmann, L.; Schulze, E.-D.; Küsel, K.
Journal Applied and Environmental Microbiology
Year 2012
Department FLOEK
Volume 78
Issue 4
Page From 1157
Page To 1167
Language englisch

Karstic caves represent one of the most important subterranean carbon storages on Earth and provide windows into the subsurface. The recent discovery of the Herrenberg Cave, Germany, gave us the opportunity to investigate the diversity and potential role of bacteria in carbonate mineral formation. Calcite was the only mineral observed by Raman spectroscopy to precipitate as stalactites from seepage water. Bacterial cells were found on the surface and interior of stalactites by confocal laser scanning microscopy. Proteobacteria dominated the microbial communities inhabiting stalactites, representing more than 70% of total 16S rRNA gene clones. Proteobacteria formed 22 to 34% of the detected communities in fluvial sediments, and a large fraction of these bacteria were also metabolically active. A total of 9 isolates, belonging to the genera Arthrobacter, Flavobacterium, Pseudomonas, Rhodococcus, Serratia, and Stenotrophomonas, grew on alkaline carbonate-precipitating medium. Two cultures with the most intense precipitate formation, Arthrobacter sulfonivorans and Rhodococcus globerulus, grew as aggregates, produced extracellular polymeric substances (EPS), and formed mixtures of calcite, vaterite, and monohydrocalcite. R. globerulus formed idiomorphous crystals with rhombohedral morphology, whereas A. sulfonivorans formed xenomorphous globular crystals, evidence for taxon-specific crystal morphologies. The results of this study highlighted the importance of combining various techniques in order to understand the geomicrobiology of karstic caves, but further studies are needed to determine whether the mineralogical biosignatures found in nutrient-rich media can also be found in oligotrophic caves.
Persistent UFZ Identifier
Rusznyák, A., Akob, D.M., Nietzsche, S., Eusterhues, K., Totsche, K.U., Neu, T.R., Frosch, T., Popp, J., Keiner, R., Geletneky, J., Katzschmann, L., Schulze, E.-D., Küsel, K. (2012):
Calcite biomineralization by bacterial isolates from the recently discovered pristine karstic Herrenberg Cave
Appl. Environ. Microb. 78 (4), 1157 - 1167