Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.reprotox.2011.12.010
Title (Primary) Transcriptional responses of zebrafish embryos exposed to potential sonic hedgehog pathway interfering compounds deviate from expression profiles of cyclopamine
Author Büttner, A.; Busch, W. ORCID logo ; Klüver, N.; Giannis, A.; Scholz, S. ORCID logo
Source Titel Reproductive Toxicology
Year 2012
Department BIOTOX
Volume 33
Issue 2
Page From 254
Page To 263
Language englisch
Keywords Hedgehog; Zebrafish; Cyclopamine; Hspb11; SANT-2; GANT-61
Abstract

The molecular responses of two small molecules, SANT-2 and GANT-61, potentially interfering with the sonic hedgehog pathway (Shh) have been studied in zebrafish embryos by microarray analysis. For both compounds and the positive reference cyclopamine previous reporter gene assays for the transcription factor Gli1 have indicated an inhibition of the hedgehog signaling pathway. In zebrafish embryos a typical phenotype (cyclopia) associated with Shh interference was only observed for cyclopamine. Furthermore, only cyclopamine led to the repression of genes specifically associated with hedgehog signaling and confirmed published microarray data. In contrast to these data hspb11 was additionally identified as the most pronounced down-regulated genes for exposure to cyclopamine. No or different effects on gene expression patterns were provoked by SANT-2 or GANT-61, respectively. Reasons for the discrepancies between cellular reporter and the zebrafish embryo assay and potential implications for the identification of compounds interfering with specific developmental pathways are discussed.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12050
Büttner, A., Busch, W., Klüver, N., Giannis, A., Scholz, S. (2012):
Transcriptional responses of zebrafish embryos exposed to potential sonic hedgehog pathway interfering compounds deviate from expression profiles of cyclopamine
Reprod. Toxicol. 33 (2), 254 - 263 10.1016/j.reprotox.2011.12.010