Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1128/AEM.06517-11 |
| Title (Primary) | Seasonal and successional influences on bacterial community composition exceed that of protozoan grazing in river biofilms |
| Author | Wey, J.K.; Jürgens, K.; Weitere, M. |
| Source Titel | Applied and Environmental Microbiology |
| Year | 2012 |
| Department | FLOEK |
| Volume | 78 |
| Issue | 6 |
| Page From | 2013 |
| Page To | 2024 |
| Language | englisch |
| Abstract | The effects of protozoa (heterotrophic flagellates and ciliates) on the morphology and community composition of bacterial biofilms were tested under natural background conditions by applying size fractionation in a river bypass system. Confocal Laser Scanning Microscopy (CLSM) was used to monitor the morphological structure of the biofilm and fingerprinting methods (Single Stranded Conformation Polymorphism [SSCP], Denaturing Gradient Gel Electrophoresis [DGGE]) were utilized to assess changes in bacterial community composition. Season and internal population dynamics had a greater influence on the bacterial biofilm than the presence of protozoa. Within this general framework, bacterial area coverage and microcolony abundance were nevertheless enhanced by the presence of ciliates (but not by the presence of flagellates). We also found that the richness of bacterial operational taxonomic units was much higher in planktonic founder communities than in the ones establishing the biofilm. Within the first two hours of colonization of an empty substrate by bacteria, the presence of flagellates additionally altered their biofilm community composition. With proceeding biofilm maturation, the number of bacterial operational taxonomic units increased when flagellates were present in high abundances. The additional presence of ciliates tended to at first reduce (days 2-7) and later increase (days 14-29) bacterial operational taxonomic unit richness. Altogether, the response of the bacterial community to protozoan grazing pressure was small compared to planktonic studies, but contradicts the assumption of a general grazing resistance of bacterial biofilms towards protozoa. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12011 |
| Wey, J.K., Jürgens, K., Weitere, M. (2012): Seasonal and successional influences on bacterial community composition exceed that of protozoan grazing in river biofilms Appl. Environ. Microb. 78 (6), 2013 - 2024 10.1128/AEM.06517-11 |
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