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Title (Primary) Surface properties and intracellular speciation revealed an original adaptive mechanism to arsenic in the acid mine drainage bio-indicator Euglena mutabilis
Author Halter, D.; Casiot, C.; Simon, S.; Heipieper, H.J.; Arsène-Ploetze, F.; Bertin, P.N.;
Journal Applied Microbiology and Biotechnology
Year 2012
Department UBT;
Volume 93
Issue 4
Language englisch;
Keywords Arsenic speciation; Euglena mutabilis; Euglena gracilis; Hydrophobicity; Fatty acid; Unsaturation index; AMD
Abstract

Euglena mutabilis is a protist ubiquitously found in extreme environments such as acid mine drainages which are often rich in arsenic. The response of E. mutabilis to this metalloid was compared to that of Euglena gracilis, a protist not found in such environments. Membrane fatty acid composition, cell surface properties, arsenic accumulation kinetics, and intracellular arsenic speciation were determined. The results revealed a modification in fatty acid composition leading to an increased membrane fluidity in both Euglena species under sublethal arsenic concentrations exposure. This increased membrane fluidity correlated to an induced gliding motility observed in E. mutabilis in the presence of this metalloid but did not affect the flagellar dependent motility of E. gracilis. Moreover, when compared to E. gracilis, E. mutabilis showed highly hydrophobic cell surface properties and a higher tolerance to water-soluble arsenical compounds but not to hydrophobic ones. Finally, E. mutabilis showed a lower accumulation of total arsenic in the intracellular compartment and an absence of arsenic methylated species in contrast to E. gracilis. Taken together, our results revealed the existence of a specific arsenical response of E. mutabilis that may play a role in its hypertolerance to this toxic metalloid.

ID 11923
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11923
Halter, D., Casiot, C., Simon, S., Heipieper, H.J., Arsène-Ploetze, F., Bertin, P.N. (2012):
Surface properties and intracellular speciation revealed an original adaptive mechanism to arsenic in the acid mine drainage bio-indicator Euglena mutabilis
Appl. Microbiol. Biotechnol. 93 (4), 1735 - 1744