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Title (Primary) Evaluation of FT-IR spectroscopy as a tool to quantify bacteria in binary mixed cultures
Author Schäwe, R.; Fetzer, I.; Tönniges, A.; Härtig, C.; Geyer, W.; Harms, H.; Chatzinotas, A.;
Journal Journal of Microbiological Methods
Year 2011
Department UMB; ANA;
Volume 86
Issue 2
Language englisch;
Keywords FT-IR spectroscopy; Mixed cultures; Rhodococcus ruber; Pseudomonas putida; Fatty acid analysis; Polyhydroxybutyrate
Abstract

Fourier-transform infrared (FT-IR) spectroscopy is known as a high-resolution method for the rapid identification of pure cultures of microorganisms. Here, we evaluated FT-IR as a method for the quantification of bacterial populations in binary mixed cultures consisting of Pseudomonas putida and Rhodococcus ruber. A calibration procedure based on Principal Component Regression was developed for estimating the ratio of the bacterial species. Data for method calibration were gained from pure cultures and artificially assembled communities of known ratios of the two member populations. Moreover, to account for physiological variability, FT-IR measurements were performed with organisms sampled at different growth phases. Measurements and data analyses were subsequently applied to growing mixed cultures revealing that growth of R. ruber was almost completely suppressed in co-culture with P. putida. Population ratios obtained by fatty acid analysis as an independent reference method were in high agreement with the FT-IR derived ratios.
ID 11688
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11688
Schäwe, R., Fetzer, I., Tönniges, A., Härtig, C., Geyer, W., Harms, H., Chatzinotas, A. (2011):
Evaluation of FT-IR spectroscopy as a tool to quantify bacteria in binary mixed cultures
J. Microbiol. Methods 86 (2), 182 - 187