Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.biortech.2011.08.055 |
| Title (Primary) | Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products |
| Author | Prieto, A.; Möder, M.; Rodil, R.; Adrian, L.
; Marco-Urrea, E. |
| Source Titel | Bioresource Technology |
| Year | 2011 |
| Department | ISOBIO; ANA |
| Volume | 102 |
| Issue | 23 |
| Page From | 10987 |
| Page To | 10995 |
| Language | englisch |
| Keywords | Fluoroquinolones; Trametes versicolor; Pharmaceuticals; Biodegradation; Laccase |
| Abstract | More than 90% of the antibiotics ciprofloxacin (CIPRO) and norfloxacin (NOR) at 2 mg L−1 were degraded by Trametes versicolor after 7 days of incubation in malt extract liquid medium. In in vitro assays with purified laccase (16.7 nkat mL−1), an extracellular enzyme excreted constitutively by this fungus, 16% of CIPRO was removed after 20 h. The addition of the laccase mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt led to 97.7% and 33.7% degradation of CIPRO and NOR, respectively. Inhibition of CIPRO and NOR degradation by the cytochrome P450 inhibitor 1-aminobenzotriazole suggests that the P450 system also plays a role in the degradation of the two antibiotics. Transformation products of CIPRO and NOR were monitored at different incubation times by triple-quadrupole and quadrupole time-of-flight mass spectrometry, and can be assigned to three different reaction pathways: (i) oxidation of the piperazinyl substituent, (ii) monohydroxylation, and (iii) formation of dimeric products. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11685 |
| Prieto, A., Möder, M., Rodil, R., Adrian, L., Marco-Urrea, E. (2011): Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products Bioresour. Technol. 102 (23), 10987 - 10995 10.1016/j.biortech.2011.08.055 |
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