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Reference Category Journals
DOI 10.1007/s00253-011-3254-y
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Title (Primary) Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98
Author Pandey, J.; Heipieper, H.J. ORCID logo ; Chauhan, A.; Arora, P.K.; Prakash, D.; Takeo, M.; Jain, R.K.
Source Titel Applied Microbiology and Biotechnology
Year 2011
Department UBT
Volume 92
Issue 3
Page From 597
Page To 606
Language englisch
Supplements https://link.springer.com/article/10.1007/s00253-011-3254-y/figures/5
https://link.springer.com/article/10.1007/s00253-011-3254-y/figures/6
https://link.springer.com/article/10.1007/s00253-011-3254-y/figures/7
Keywords Chlorinated nitroaromatic compounds; Burkholderia; Aerobic degradation
Abstract

Burkholderia sp. strain SJ98 (DSM 23195) was previously isolated and characterized for degradation and co-metabolic transformation of a number nitroaromatic compounds. In the present study, we evaluated its metabolic activity on chlorinated nitroaromatic compounds (CNACs). Results obtained during this study revealed that strain SJ98 can degrade 2-chloro-4-nitrophenol (2C4NP) and utilize it as sole source of carbon, nitrogen, and energy under aerobic conditions. The cells of strain SJ98 removed 2C4NP from the growth medium with sequential release of nearly stoichiometric amounts of chloride and nitrite in culture supernatant. Under aerobic degradation conditions, 2C4NP was transformed into the first intermediate that was identified as p-nitrophenol by high-performance liquid chromatography, LCMS-TOF, and GC-MS analyses. This transformation clearly establishes that the degradation of 2C4NP by strain SJ98 is initiated by “reductive dehalogenation”; an initiation mechanism that has not been previously reported for microbial degradation of CNAC under aerobic conditions.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11646
Pandey, J., Heipieper, H.J., Chauhan, A., Arora, P.K., Prakash, D., Takeo, M., Jain, R.K. (2011):
Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98
Appl. Microbiol. Biotechnol. 92 (3), 597 - 606 10.1007/s00253-011-3254-y