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Title (Primary) Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil
Author Llado, S.; Jiménez, N.; Viñas, M.; Solanas, A.M.;
Journal Biodegradation
Year 2009
Department ISOBIO;
Volume 20
Issue 5
Language englisch;
Keywords Polycyclic aromatic hydrocarbons; Biodegradation; Bioavailability; Bioremediation; Pyrene; Chrysene; 16SrRNA; DGGE
Abstract

A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.

ID 11323
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11323
Llado, S., Jiménez, N., Viñas, M., Solanas, A.M. (2009):
Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil
Biodegradation 20 (5), 593 - 601