Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1007/s10532-009-9247-1 |
Document | Shareable Link |
Title (Primary) | Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil |
Author | Llado, S.; Jiménez, N.; Viñas, M.; Solanas, A.M. |
Source Titel | Biodegradation |
Year | 2009 |
Department | ISOBIO |
Volume | 20 |
Issue | 5 |
Page From | 593 |
Page To | 601 |
Language | englisch |
Keywords | Polycyclic aromatic hydrocarbons; Biodegradation; Bioavailability; Bioremediation; Pyrene; Chrysene; 16SrRNA; DGGE |
Abstract |
A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11323 |
Llado, S., Jiménez, N., Viñas, M., Solanas, A.M. (2009): Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil Biodegradation 20 (5), 593 - 601 10.1007/s10532-009-9247-1 |