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Category Text Publication
Reference Category Journals
DOI 10.1007/s10532-009-9319-2
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Title (Primary) Enhanced decolourization of Direct Red-80 dye by the white rot fungus Phanerochaete chrysosporium employing sequential design of experiments
Author Singh, S.; Pakshirajan, K.; Daverey, A.
Source Titel Biodegradation
Year 2010
Department BIOENERGIE
Volume 21
Issue 4
Page From 501
Page To 511
Language englisch
Keywords Direct Red-80; Phanerochaete chrysosporium; Lignin peroxidase; Dye decolourization; Azo dyes
Abstract

Decolourization of Direct Red 80 (DR-80) by the white rot fungus Phanerochaete chrysosporium MTCC 787 was investigated employing sequential design of experiments. Media components for growing the white rot fungus were first screened using Plackett-Burman design and then optimized using response surface methodology (RSM), which resulted in enhancement in the efficiency of dye removal by the fungus. For determining the effect of media constituents on the dye removal, both percent dye decolourization and specific dye removal due to maximum enzyme activity were chosen as the responses from the experiments, and the media constituents glucose, veratryl alcohol, KH2PO4, CaCl2 and MgSO4 were screened to be the most effective with P values less than 0.05. Central composite design (CCD) followed by RSM in the optimization study revealed the following optimum combinations of the screened media constituents: glucose, 11.9 g l−1; veratryl alcohol, 12.03 mM; KH2PO4, 23.08 g l−1; CaCl2, 2.4 g l−1; MgSO4, 10.47 g l−1. At the optimum settings of the media constituents, complete dye decolourization (100% removal efficiency) and a maximum specific dye removal due to lignin peroxidase enzyme of 0.24 mg U−1 by the white rot fungus were observed.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11210
Singh, S., Pakshirajan, K., Daverey, A. (2010):
Enhanced decolourization of Direct Red-80 dye by the white rot fungus Phanerochaete chrysosporium employing sequential design of experiments
Biodegradation 21 (4), 501 - 511 10.1007/s10532-009-9319-2