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Title (Primary) Comparison of naphthalene bioavailability determined by whole-cell biosensing and availability determined by extraction with Tenax
Author Kohlmeier, S.; Mancuso, M.; Deepthike, U.; Tecon, R.; van der Meer, J.R.; Harms, H.; Wells, M.;
Journal Environmental Pollution
Year 2008
Department UMB;
Volume 156
Issue 3
Language englisch;
Keywords Bioavailability; Whole-cell biosensor; Contaminants; Pollution; Risk assessment
Abstract A rapid biological method for the determination of the bioavailability of naphthalene was developed and its value as an alternative to extraction-based chemical approaches demonstrated. Genetically engineered whole-cell biosensors are used to determine bioavailable naphthalene and their responses compared with results from Tenax extraction and chemical analysis. Results show a 1:1 correlation between biosensor results and chemical analyses for naphthalene-contaminated model materials and sediments, but the biosensor assay is much faster. This work demonstrates that biosensor technology can perform as well as standard chemical methods, though with some advantages including the inherent biological relevance of the response, rapid response time, and potential for field deployment. A survey of results from this work and the literature shows that bioavailability under non-equilibrium conditions nonetheless correlates well with Koc or K. A rationale is provided wherein chemical resistance is speculated to be operative.Whole-cell biosensing and mild extraction followed by chemical analysis quantify bioavailable naphthalene in sediment materials equally well.
ID 1118
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1118
Kohlmeier, S., Mancuso, M., Deepthike, U., Tecon, R., van der Meer, J.R., Harms, H., Wells, M. (2008):
Comparison of naphthalene bioavailability determined by whole-cell biosensing and availability determined by extraction with Tenax
Environ. Pollut. 156 (3), 803 - 808