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Title (Primary) Development of an enantiomer-specific stable carbon isotope analysis (ESIA) method for assessing the fate of a-hexachlorocyclo-hexane in the environment
Author Badea, S.L.; Vogt, C.; Gehre, M.; Fischer, A.; Danet, A.-F.; Richnow, H.-H.;
Journal Rapid Communications in Mass Spectrometry
Year 2011
Department ISOBIO;
Volume 25
Issue 10
Language englisch;
Abstract a-Hexachlorocyclohexane (a-HCH) is the only chiral isomer of the eight 1,2,3,4,5,6-HCHs and we have developed an enantiomer-specific stable carbon isotope analysis (ESIA) method for the evaluation of its fate in the environment. The carbon isotope ratios of the a-HCH enantiomers were determined for a commercially available a-HCH sample using a gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) system equipped with a chiral column. The GC-C-IRMS measurements revealed d-values of -32.5?±?0.8‰ and -32.3?±?0.5‰ for (-) a-HCH and (+) a-HCH, respectively. The isotope ratio of bulk a-HCH was estimated to be -32.4?±?0.6‰ which was in accordance with the d-values obtained by GC-C-IRMS (-32.7?±?0.2‰) and elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) of the bulk a-HCH (-32.1?±?0.1‰). The similarity of the isotope ratio measurements of bulk a-HCH by EA-IRMS and GC-C-IRMS indicates the accuracy of the chiral GC-C-IRMS method. The linearity of the a-HCH ESIA method shows that carbon isotope ratios can be obtained for a signal size above 100?mV. The ESIA measurements exhibited standard deviations (2s) that were mostly < ±?0.5‰. In order to test the chiral GC-C-IRMS method, the isotope compositions of individual enantiomers in biodegradation experiments of a-HCH with Clostridium pasteurianum and samples from a contaminated field site were determined. The isotopic compositions of the a-HCH enantiomers show a range of enantiomeric and isotope patterns, suggesting that enantiomeric and isotope fractionation can serve as an indicator for biodegradation and source characterization of a-HCH in the environment.
ID 11069
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=11069
Badea, S.L., Vogt, C., Gehre, M., Fischer, A., Danet, A.-F., Richnow, H.-H. (2011):
Development of an enantiomer-specific stable carbon isotope analysis (ESIA) method for assessing the fate of a-hexachlorocyclo-hexane in the environment
Rapid Commun. Mass Spectrom. 25 (10), 1363 - 1372