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Title (Primary) KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis
Author Cialfi, S.; Uccelletti, D.; Carducci, A.; W├ęsolowski-Louvel, M.; Mancini, P.; Heipieper, H.J.; Saliola, M.;
Journal Microbiology-SGM
Year 2011
Department UBT;
Volume 157
Issue 5
Language englisch;
Keywords AA, antimycin A; CWI, cell wall integrity; FACS, fluorescence-activated cell sorting; MAPK, mitogen-activated protein kinase

In Saccharomyces cerevisiae, HSL1 (NIK1) encodes a serine-threonine protein kinase involved in cell cycle control and morphogenesis. Deletion of its putative orthologue in Kluyveromyces lactis, KlHSL1, gives rise to sensitivity to the respiratory inhibitor antimycin A (AA). Resistance to AA on glucose (Rag+ phenotype) is associated with genes (RAG) required for glucose metabolism/glycolysis. To understand the relationship between RAG and KlHSL1, rag and Klhsl1 mutant strains were investigated. The analysis showed that all the mutants contained a phosphorylated form of Hog1 and displayed an inability to synthesize/accumulate glycerol as a compatible solute. In addition, rag mutants also showed alterations in both cell wall and membrane fatty acids. The pleiotropic defects of these strains indicate that a common pathway regulates glucose utilization and stress response mechanisms, suggesting impaired adaptation of the plasma membrane/cell wall during the respiratory–fermentative transition. KlHsl1 could be the link between these adaptive pathways and the morphogenetic checkpoint.

ID 10970
Persistent UFZ Identifier
Cialfi, S., Uccelletti, D., Carducci, A., W├ęsolowski-Louvel, M., Mancini, P., Heipieper, H.J., Saliola, M. (2011):
KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis
Microbiology-(UK) 157 (5), 1509 - 1518