Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1099/mic.0.044040-0 |
Title (Primary) | KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis |
Author | Cialfi, S.; Uccelletti, D.; Carducci, A.; Wésolowski-Louvel, M.; Mancini, P.; Heipieper, H.J. ; Saliola, M. |
Source Titel | Microbiology-SGM |
Year | 2011 |
Department | UBT |
Volume | 157 |
Issue | 5 |
Page From | 1509 |
Page To | 1518 |
Language | englisch |
Keywords | AA, antimycin A; CWI, cell wall integrity; FACS, fluorescence-activated cell sorting; MAPK, mitogen-activated protein kinase |
Abstract |
In Saccharomyces cerevisiae, HSL1 (NIK1) encodes a serine-threonine protein kinase involved in cell cycle control and morphogenesis. Deletion of its putative orthologue in Kluyveromyces lactis, KlHSL1, gives rise to sensitivity to the respiratory inhibitor antimycin A (AA). Resistance to AA on glucose (Rag+ phenotype) is associated with genes (RAG) required for glucose metabolism/glycolysis. To understand the relationship between RAG and KlHSL1, rag and Klhsl1 mutant strains were investigated. The analysis showed that all the mutants contained a phosphorylated form of Hog1 and displayed an inability to synthesize/accumulate glycerol as a compatible solute. In addition, rag mutants also showed alterations in both cell wall and membrane fatty acids. The pleiotropic defects of these strains indicate that a common pathway regulates glucose utilization and stress response mechanisms, suggesting impaired adaptation of the plasma membrane/cell wall during the respiratory–fermentative transition. KlHsl1 could be the link between these adaptive pathways and the morphogenetic checkpoint. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10970 |
Cialfi, S., Uccelletti, D., Carducci, A., Wésolowski-Louvel, M., Mancini, P., Heipieper, H.J., Saliola, M. (2011): KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis Microbiology-(UK) 157 (5), 1509 - 1518 10.1099/mic.0.044040-0 |