Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.jbiotec.2011.03.013 |
| Title (Primary) | Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica |
| Author | Moeller, L.; Grünberg, M.; Zehnsdorf, A.; Aurich, A.; Bley, T.; Strehlitz, B. |
| Source Titel | Journal of Biotechnology |
| Year | 2011 |
| Department | UBZ |
| Volume | 153 |
| Issue | 3-4 |
| Page From | 133 |
| Page To | 137 |
| Language | englisch |
| Keywords | citric acid; Yarrowia lipolytica; glucose biosensor; biosensor online control; semicontinuous fermentation; repeated fed-batch |
| Abstract | Biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain Yarrowia lipolytica H222 with glucose as the carbon source. The application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. Ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of the glucose biosensor in a long-time experiment. In the course of this fermentation with a duration of 553 h, a slight loss of productivity from 1.4 g/(L*h) to 1.1 g/(L*h) and of selectivity for citric acid from 91 % to 88 % was observed. The glucose biosensor provided 6,227 measurements without any loss of activity. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10931 |
| Moeller, L., Grünberg, M., Zehnsdorf, A., Aurich, A., Bley, T., Strehlitz, B. (2011): Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica J. Biotechnol. 153 (3-4), 133 - 137 10.1016/j.jbiotec.2011.03.013 |
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