Publication Details |
| Reference Category | Journals |
| DOI / URL | link |
| Document | Shareable Link |
| Title (Primary) | Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters |
| Author | Kalkhof, S.; Sinz, A. |
| Journal | Analytical and Bioanalytical Chemistry |
| Year | 2008 |
| Department | PROTEOM |
| Volume | 392 |
| Issue | 1-2 |
| Page From | 305 |
| Page To | 312 |
| Language | englisch |
| Supplements | https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-008-2231-5/MediaObjects/216_2008_2231_MOESM1_ESM.pdf |
| Keywords | Chemical cross-linking; Amine-reactive cross-linkers; Mass spectrometry |
| Abstract | In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.
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| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1083 |
| Kalkhof, S., Sinz, A. (2008): Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters Anal. Bioanal. Chem. 392 (1-2), 305 - 312 |
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