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Title (Primary) Photosynthetic energy conversion in the diatom Phaeodactylum tricornutum. Measuring by calorimetry, oxygen evolution and pulse-amplitude modulated fluorescence
Author Oroszi, S.; Jakob, T.; Wilhelm, C.; Harms, H.; Maskow, T.;
Journal Journal of Thermal Analysis and Calorimetry
Year 2011
Department UMB;
Volume 104
Issue 1
Language englisch;
Keywords Calorimetry; Diatom; Fluorescence; Oxygen evolution; Photosynthesis
Abstract Isothermal microcalorimetry can be used to investigate the photosynthetic energy conversion of autotrophic organisms. In this study, for the first time a diatom alga was used to compare the calorimetrically measured heat flux with measurements of the photosynthetic performance by oxygen evolution and pulse-amplitude modulated fluorescence. The presented experimental setup proved suitable to compare calorimetric data with those of conventional methods of the determination of photosynthesis rates. Special attention was paid to the contribution of energy dissipation via non-photochemical quenching (NPQ) of chlorophyll fluorescence to the metabolic energy balance. This was achieved by a combination of different light conditions and the use of an inhibitor of NPQ. Although NPQ is an important photoprotective mechanism in diatoms, the inhibition of NPQ resulted in an activation of alternative, energy dissipating pathways for absorbed radiation which completely compensated for the fraction of energy dissipation by NPQ.
ID 10810
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10810
Oroszi, S., Jakob, T., Wilhelm, C., Harms, H., Maskow, T. (2011):
Photosynthetic energy conversion in the diatom Phaeodactylum tricornutum. Measuring by calorimetry, oxygen evolution and pulse-amplitude modulated fluorescence
J. Therm. Anal. Calorim. 104 (1), 223 - 231