Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1021/pr100723d |
| Title (Primary) | DIGE-based protein expression analysis of B[a]P-exposed hepatoma cells reveals a complex stress response including alterations in oxidative stress, cell cycle control, and cytoskeleton motility at toxic and subacute concentrations |
| Author | Dautel, F.; Kalkhof, S.; Trump, S.; Michaelson, J.; Beyer, A.; Lehmann, I.; von Bergen, M. |
| Source Titel | Journal of Proteome Research |
| Year | 2011 |
| Department | IMMU; PROTEOM |
| Volume | 10 |
| Issue | 2 |
| Page From | 379 |
| Page To | 393 |
| Language | englisch |
| Keywords | benzo[a]pyrene (B[a]P); aryl hydrocarbon receptor (Ahr); oxidative stress; human protein reference database (HPRD); protein expression analysis; DIGE |
| Abstract | Although the effects of high concentrations of the carcinogen benzo[a]pyrene (B[a]P) have been studied extensively, little is known about its effects at subacute toxic concentrations, which are typical for environmental pollutants. We exposed murine Hepa1c1c7 cells to a toxic concentration (5 µM) and a subacute concentration (50 nM) of B[a]P over a period of 2-24 h to differentiate between acute and pseudochronic effects and conducted a time-course analysis of B[a]P-influenced protein expression by DIGE. In total, a set of 120 spots were found to be significantly altered due to B[a]P exposure of which 112 were subsequently identified by mass spectrometry. Clustering and principal component analysis were conducted to identify sets of proteins responding in a concerted manner to the exposure. Our results indicate an immediate response to the contaminant at the protein level and demonstrate that B[a]P exposure alters the cellular response by disturbing proteins involved in oxidative stress, cell cycle regulation, apoptosis, and cytoskeleton organization. Furthermore, network analysis of protein-protein interactions revealed a complex network of interacting, B[a]P-regulated proteins mostly belonging to the cytoskeleton organization and several signal transduction pathways. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10734 |
| Dautel, F., Kalkhof, S., Trump, S., Michaelson, J., Beyer, A., Lehmann, I., von Bergen, M. (2011): DIGE-based protein expression analysis of B[a]P-exposed hepatoma cells reveals a complex stress response including alterations in oxidative stress, cell cycle control, and cytoskeleton motility at toxic and subacute concentrations J. Proteome Res. 10 (2), 379 - 393 10.1021/pr100723d |
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