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Title (Primary) Passive dosing: an approach to control mutagen exposure in the Ames fluctuation test
Author Bougeard, C.; Gallampois, C.; Brack, W.;
Journal Chemosphere
Year 2011
Department WANA;
Volume 83
Issue 4
Language englisch;
Keywords Exposure; Mutagenicity; Partition-based dosing; Salmonella; Nitro-PAH; Amino-PAH
Abstract One of the major challenges for mutagenicity assessment of environmental samples and individual compounds for example in the Ames fluctuation test (AFT) is the establishment and control of a well defined exposure concentration. Thus, a combination of passive dosing with silicone O-rings (SRs) together with an analytical confirmation of the freely dissolved concentration (FDC) is presented. FDCs are often determined with a combination of solid phase micro-extraction (SPME) with gas chromatography (GC). For compounds with poor performance in GC, a high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of bi-distilled water dosed with identically loaded SRs is suggested to avoid interference of the bacterial culture. The approach was tested for six amino-, nitro-, and keto-substituted polycyclic aromatic compounds with a log KOW range of 2.5-5.1 without metabolic activation. The method provided reliable concentration-effect relationships and freely dissolved 50% effect concentrations (DEC50) 3-33 times lower than nominal effect concentrations (NEC50) derived in parallel solvent-dosed AFT. Partition coefficients and NEC50/DEC50 ratios were well correlated with lipophilicity.
ID 10721
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10721
Bougeard, C., Gallampois, C., Brack, W. (2011):
Passive dosing: an approach to control mutagen exposure in the Ames fluctuation test
Chemosphere 83 (4), 409 - 414