Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.chemosphere.2010.12.087
Title (Primary) Passive dosing: an approach to control mutagen exposure in the Ames fluctuation test
Author Bougeard, C.; Gallampois, C.; Brack, W.
Source Titel Chemosphere
Year 2011
Department WANA
Volume 83
Issue 4
Page From 409
Page To 414
Language englisch
Keywords Exposure; Mutagenicity; Partition-based dosing; Salmonella; Nitro-PAH; Amino-PAH
Abstract One of the major challenges for mutagenicity assessment of environmental samples and individual compounds for example in the Ames fluctuation test (AFT) is the establishment and control of a well defined exposure concentration. Thus, a combination of passive dosing with silicone O-rings (SRs) together with an analytical confirmation of the freely dissolved concentration (FDC) is presented. FDCs are often determined with a combination of solid phase micro-extraction (SPME) with gas chromatography (GC). For compounds with poor performance in GC, a high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of bi-distilled water dosed with identically loaded SRs is suggested to avoid interference of the bacterial culture. The approach was tested for six amino-, nitro-, and keto-substituted polycyclic aromatic compounds with a log KOW range of 2.5-5.1 without metabolic activation. The method provided reliable concentration-effect relationships and freely dissolved 50% effect concentrations (DEC50) 3-33 times lower than nominal effect concentrations (NEC50) derived in parallel solvent-dosed AFT. Partition coefficients and NEC50/DEC50 ratios were well correlated with lipophilicity.
Persistent UFZ Identifier
Bougeard, C., Gallampois, C., Brack, W. (2011):
Passive dosing: an approach to control mutagen exposure in the Ames fluctuation test
Chemosphere 83 (4), 409 - 414 10.1016/j.chemosphere.2010.12.087