Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.scitotenv.2010.06.048
Title (Primary) Responses of soil microbial communities to weak electric fields
Author Wick, L.Y.; Buchholz, F.; Fetzer, I.; Kleinsteuber, S. ORCID logo ; Härtig, C.; Shi, L.; Miltner, A. ORCID logo ; Harms, H.; Pucci, G.N.
Source Titel Science of the Total Environment
Year 2010
Department UMB; UBT
Volume 408
Issue 20
Page From 4886
Page To 4893
Language englisch
Keywords Bioremediation; Electrokinetics; Microorganism; Phospholipid fatty acids; T-RFLP; Microbial community
Abstract Electrokinetically stimulated bioremediation of soils (electro-bioremediation) requires that the application of weak electric fields has no negative effect on the contaminant degrading microbial communities. This study evaluated the hypothesis that weak direct electric current (DC) fields per se do not negatively influence the physiology and composition of soil microbial communities given that secondary electrokinetic phenomena such as soil pH changes and temperatures are minimized. Mildly buffered, water-saturated laboratory mesocosms with agricultural soil were subjected for 34 days to a constant electric field (X = 1.4 V cm- 1; J 1.0 mA cm- 2) and the spatiotemporal changes of soil microbial communities assessed by fingerprints of phospholipids fatty acids (PLFA) and terminal restriction fragment length polymorphisms (T-RFLP) of bacterial 16S rRNA genes. DC-induced electrolysis of the pore water led to pH changes (< 1.5 pH units) in the immediate vicinity of the electrodes and concomitant distinct soil microbial community changes. By contrast, DC-treated bulk soil distant to the electrodes showed no pH changes and developed similar PLFA- and T-RFLP-fingerprints as control soil in the absence of DC. Our data suggest that the presence of an electric field, if suitably applied, will not influence the composition and physiology of soil microbial communities and hence not affect their potential to biodegrade contaminants.
Persistent UFZ Identifier
Wick, L.Y., Buchholz, F., Fetzer, I., Kleinsteuber, S., Härtig, C., Shi, L., Miltner, A., Harms, H., Pucci, G.N. (2010):
Responses of soil microbial communities to weak electric fields
Sci. Total Environ. 408 (20), 4886 - 4893 10.1016/j.scitotenv.2010.06.048