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Title (Primary) High-throughput generation of selected reaction-monitoring assays for proteins and proteomes
Author Picotti, P.; Rinner, O.; Stallmach, R.; Dautel, F.; Farrah, T.; Domon, B.; Wenschuh, H.; Aebersold, R.;
Journal Nature Methods
Year 2010
Department PROTEOM;
Volume 7
Language englisch;
Supplements https://media.nature.com/original/nature-assets/nmeth/journal/v7/n1/extref/nmeth.1408-S1.pdf
Abstract Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
ID 10391
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10391
Picotti, P., Rinner, O., Stallmach, R., Dautel, F., Farrah, T., Domon, B., Wenschuh, H., Aebersold, R. (2010):
High-throughput generation of selected reaction-monitoring assays for proteins and proteomes
Nat. Methods 7 , 43 - 46