Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1038/nmeth.1408 |
| Document | Shareable Link |
| Title (Primary) | High-throughput generation of selected reaction-monitoring assays for proteins and proteomes |
| Author | Picotti, P.; Rinner, O.; Stallmach, R.; Dautel, F.; Farrah, T.; Domon, B.; Wenschuh, H.; Aebersold, R. |
| Source Titel | Nature Methods |
| Year | 2010 |
| Department | PROTEOM |
| Volume | 7 |
| Page From | 43 |
| Page To | 46 |
| Language | englisch |
| Supplements | https://media.nature.com/original/nature-assets/nmeth/journal/v7/n1/extref/nmeth.1408-S1.pdf |
| Abstract | Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10391 |
| Picotti, P., Rinner, O., Stallmach, R., Dautel, F., Farrah, T., Domon, B., Wenschuh, H., Aebersold, R. (2010): High-throughput generation of selected reaction-monitoring assays for proteins and proteomes Nat. Methods 7 , 43 - 46 10.1038/nmeth.1408 |
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