Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.chroma.2010.07.049
Title (Primary) Determination of alkylphenols and 17β-estradiol in fish homogenate. Extraction and clean-up strategies
Author Navarro, P.; Bustamante, J.; Vallejo, A.; Prieto, A.; Usobiaga, A.; Arrasate, S.; Anakabe, E.; Puy-Azurmendi, E.; Zuloaga, O.
Source Titel Journal of Chromatography A
Year 2010
Department ANA
Volume 1217
Issue 38
Page From 5890
Page To 5895
Language englisch
Keywords Nonylphenols; Octylphenols; 17ß-Estradiol; Fish homogenate; Focused-ultrasound liquid extraction; Microwave-assisted solvent extraction; Clean-up; Solid phase extraction; Gel permeation chromatography
Abstract The determination of target analytes such as nonyl- and octylphenols and 17ß-estradiol in fish homogenate require of solid-liquid extraction step. In this work microwave-assisted extraction (MAE) and focused-ultrasound liquid extraction (FUSLE) were studied as two different alternatives for extraction of the target compounds in zebrafish (Danio rerio) homogenate. In this work solid phase extraction (SPE) using 5-g and 10-g Florisil cartridges and gel permeation chromatography (GPC) were studied for the clean-up of the MAE and FUSLE extracts due to the non-selective extraction step. Although good recoveries were obtained both for SPE (106% and 126% range) and GPC (79% and 100% range) clean-up procedures, cleaner chromatograms were obtained after SPE and finally 5-g Florisil cartridges were tested since no improvement was observed when 10-g Florisil cartridges were used. Under optimized clean-up conditions, MAE and FUSLE provided comparable results for 4nOP and NP, while more accurate results were obtained for 4tOP and E2 after FUSLE. Finally, the method was applied to the determination of alkylphenols and 17ß-estradiol in zebrafish homogenate that had been exposed to known concentrations of the target analytes. In the case of alkylphenols two different isomers of nonyl- and octylphenol (4-(3',6'-dimethyl-3'-hepthyl)phenol, 363-NP, and 4-(3'-methyl-3'-hepthyl)phenol, 33-OP) were studied.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10350
Navarro, P., Bustamante, J., Vallejo, A., Prieto, A., Usobiaga, A., Arrasate, S., Anakabe, E., Puy-Azurmendi, E., Zuloaga, O. (2010):
Determination of alkylphenols and 17β-estradiol in fish homogenate. Extraction and clean-up strategies
J. Chromatogr. A 1217 (38), 5890 - 5895 10.1016/j.chroma.2010.07.049